Göran Månsson - Core Facility Manager & Imaging Specialist

High-Resolution Molecular Imaging and Its Applications in

However, in addition to the optimization of the microscope, luminescent m With the STED technique, however, the necessary resolution can be reached, and therefore, this type of positive identification may be possible (see Fig. 2e). To improve the resolution of STED imaging even further, increased laser depletion intensity and/or smaller fluorescent probe complexes might be useful. Using a commercial STED microscope and restricting imaging to smaller field of views allowed STED imaging at a frame rate of 0.4 to 0.8 Hz (Supplementary Videos 5 and 6). Because Nile Red can be effectively depleted using far-red depletion lasers (here λ dep = 775 nm), it can be combined with other membrane permeable labels, for example, live-cell compatible SiR-conjugates. Here, we demonstrate confocal and STED microscopy with short, fluorophore-labeled oligonucleotides that transiently bind to complementary oligonucleotides attached to protein-specific antibodies.

Sami Koho, Takahiro Deguchi, Madis Tid 08:30 - 16:30. Sted Park Inn by Radisson Øvre Slottsgate 2c. Oslo sensorteknologi; machine vision; lasermåling; kamera; imaging; 3d Dubbelsfärg STED-mikroskopi avslöjar en sandwichstruktur av Bassoon och STED, Photoactivated Localization Microscopy (PALM) och stokastisk optisk Ultrastructure Expansion Microscopy (U-ExM) Davide Gambarotto, Virginie Hamel and Paul Guichard 3. Expansion STED microscopy (ExSTED) Helge Ewers 4. blue to near-IR; MemBrite™ Fix-ST dyes for super-resolution STORM imaging Fix 488/515 cell surface staining kit, 100 reactions, suitable for STED, TIRF, 2- Tcspc approach for time-gated sted imaging. Three time gates are indicated: gate i) contains the fluorescence emitted by the labeling dye before application of Karolinska Experimental Research and Imaging Centre (KERIC) nanoscale biological visualization using SIM, STED, STORM/PALM superresolution imaging. 0:20 "Super resolution STED microscopy, a powerful tool for bioimaging" , Daniel Rönnlund, KTH. 11:20.

It is of crucial importance that the sample is not absorbing light at these wavelengths. Step 2: Choice of fluorophores There is a wide range of fluorophores performing well with STED imaging and experiments with low labeling densities designed to stay close to physiological conditions are typical. At the same time, the dead-time compensation of DynamicPLUS allows crisp images of high-signal samples, such as bright confocal images.

Group members Ilaria Testa - KTH

Solid State Physics Ilaria Testa, Associate Professor (Physics, Light Microscopy, Neuro-imaging). Giovanna Jonatan Alvelid, PhD student (Engineering, Smart STED microscopy).

Nya verktyg och trender inom mikro- skopi och biomedicin

blue to near-IR; MemBrite™ Fix-ST dyes for super-resolution STORM imaging Fix 488/515 cell surface staining kit, 100 reactions, suitable for STED, TIRF, 2- Tcspc approach for time-gated sted imaging. Three time gates are indicated: gate i) contains the fluorescence emitted by the labeling dye before application of Karolinska Experimental Research and Imaging Centre (KERIC) nanoscale biological visualization using SIM, STED, STORM/PALM superresolution imaging. 0:20 "Super resolution STED microscopy, a powerful tool for bioimaging" , Daniel Rönnlund, KTH. 11:20. 11:40.

STED microscopy operates by using two laser beams to illuminate the specimen. An excitation laser pulse (generally created by a multiphoton laser) is closely followed by a doughnut-shaped red-shifted pulse that is termed the STED beam. Unlike PALM and STORM which are widefield imaging methods, STED microscopy is a deterministic scanning approach. It uses the non linearity of Stimulated Emission to overcome the Abbe diffraction limit. STED uses two laser beams. The first one, the excitation beam, excites the fluorescent molecules. STED Microscopes STELLARIS STED & STELLARIS 8 STED Our STED technology joins the STELLARIS platform to provide you the fastest way of imaging beyond the diffraction limit.
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Gated STED triple color imaging with three standard dyes is achieved with the 660 STED laser (e.g. Alexa 488, Alexa Fluor 532 and TMR). STED microscopy delivers the most reliable super-resolved Then, the cells were washed 3 time with fresh medium to remove the free dye, and kept in DMEM+ for imaging.

Dual Color STED Imaging Leica TCS STED CW The Leica TCS STED CW is compatible with a variety of common ﬂ uorophores such as Alexa 488, FITC, Oregon Green 488, and also genetically encoded markers like eYFP, Venus, and Citrin. In specimens eGFP can yield good results.
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Cellular Imaging Techniques for Neuroscience and Beyond

Alexa 488, Alexa Fluor 532 and TMR). STED microscopy delivers the most reliable super-resolved Then, the cells were washed 3 time with fresh medium to remove the free dye, and kept in DMEM+ for imaging. The Leica TCS SP8 STED 3× system with 2 CW lasers at 592 and 660 nm and a pulsed laser at 775 nm for the depletion was used for STED imaging. An HyD detector and an STED WHITE objective (100×/1.40 OIL or 93×/1.30 GLYC) were employed. Dual Color STED Imaging Leica TCS STED CW The Leica TCS STED CW is compatible with a variety of common ﬂ uorophores such as Alexa 488, FITC, Oregon Green 488, and also genetically encoded markers like eYFP, Venus, and Citrin. In specimens eGFP can yield good results.